Biotransformation of linear alkylbenzene sulfonate (LAS) by Phanerochaete chrysosporium: oxidation of alkyl side-chain.
Identifieur interne : 000A52 ( Main/Exploration ); précédent : 000A51; suivant : 000A53Biotransformation of linear alkylbenzene sulfonate (LAS) by Phanerochaete chrysosporium: oxidation of alkyl side-chain.
Auteurs : J S Yadav [États-Unis] ; D L Lawrence ; B A Nuck ; T W Federle ; C A ReddySource :
- Biodegradation [ 0923-9820 ] ; 2001.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Acides sulfoniques, Phanerochaete.
- Biotransformation, Oxydoréduction, Spectrométrie de masse ESI.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Sulfonic Acids.
- metabolism : Phanerochaete.
- Biotransformation, Oxidation-Reduction, Spectrometry, Mass, Electrospray Ionization.
Abstract
The white rot fungus Phanerochaete chrysosporium, which generally mineralizes substituted aromatics to CO2, transformed linear alkylbenzene sulfonate (LAS) surfactants mainly at their alkyl side chain. Degradation of LAS was evidenced by a zone of clearing on LAS-containing agar plates and colorimetric analysis of liquid cultures. Disappearance of LAS was virtually complete within 10 days in low nitrogen (2.4 mM N), high nitrogen (24 mM N) and malt extract (ME) liquid media. After 5 days of incubation in ME medium, transformation of LAS was complete at concentrations < or = 4 mg l(-1), but decreased at higher concentrations. The LAS degradation was not dependent on lignin peroxidases (LiPs) and manganese-dependent peroxidases (MnPs). Mineralization of 14C-ring-LAS to 14CO2 by P. chrysosporium was < 1% regardless of the culture conditions used. Thin layer chromatography and mass spectral analyses indicated that P. chrysosporium transformed LAS to sulfophenyl carboxylates (SPCs) through oxidative shortening of the alkyl side-chains. While LAS disappearance in the cultures was not dependent on LiPs and MnPs, transformation of the parent LAS moieties to SPCs was more extensive in low N medium that favors expression of these enzymes. The SPCs produced in LN cultures were shorter in chain- length than those produced in ME cultures. Also there was a notable shift in the relative abundance of odd and even chain length metabolites compared to the starting LAS particularly in the low N cultures suggesting the possible involvement of processes other than or in addition to beta-oxidation in the chain-shortening process.
DOI: 10.1023/a:1015056530264
PubMed: 12051650
Affiliations:
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Lawrence</name></author><author><name sortKey="Nuck, B A" sort="Nuck, B A" uniqKey="Nuck B" first="B A" last="Nuck">B A Nuck</name></author><author><name sortKey="Federle, T W" sort="Federle, T W" uniqKey="Federle T" first="T W" last="Federle">T W Federle</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:12051650</idno><idno type="pmid">12051650</idno><idno type="doi">10.1023/a:1015056530264</idno><idno type="wicri:Area/Main/Corpus">000999</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000999</idno><idno type="wicri:Area/Main/Curation">000999</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000999</idno><idno type="wicri:Area/Main/Exploration">000999</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biotransformation of linear alkylbenzene sulfonate (LAS) by Phanerochaete chrysosporium: oxidation of alkyl side-chain.</title><author><name sortKey="Yadav, J S" sort="Yadav, J S" uniqKey="Yadav J" first="J S" last="Yadav">J S Yadav</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, and NSF Center for Microbial Ecology, Michigan State University, East Lansing 48824-1101, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, and NSF Center for Microbial Ecology, Michigan State University, East Lansing 48824-1101</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Lawrence, D L" sort="Lawrence, D L" uniqKey="Lawrence D" first="D L" last="Lawrence">D L Lawrence</name></author><author><name sortKey="Nuck, B A" sort="Nuck, B A" uniqKey="Nuck B" first="B A" last="Nuck">B A Nuck</name></author><author><name sortKey="Federle, T W" sort="Federle, T W" uniqKey="Federle T" first="T W" last="Federle">T W Federle</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></analytic><series><title level="j">Biodegradation</title><idno type="ISSN">0923-9820</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotransformation (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Phanerochaete (metabolism)</term><term>Spectrometry, Mass, Electrospray Ionization (MeSH)</term><term>Sulfonic Acids (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides sulfoniques (métabolisme)</term><term>Biotransformation (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Phanerochaete (métabolisme)</term><term>Spectrométrie de masse ESI (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sulfonic Acids</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides sulfoniques</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biotransformation</term><term>Oxidation-Reduction</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biotransformation</term><term>Oxydoréduction</term><term>Spectrométrie de masse ESI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The white rot fungus Phanerochaete chrysosporium, which generally mineralizes substituted aromatics to CO2, transformed linear alkylbenzene sulfonate (LAS) surfactants mainly at their alkyl side chain. Degradation of LAS was evidenced by a zone of clearing on LAS-containing agar plates and colorimetric analysis of liquid cultures. Disappearance of LAS was virtually complete within 10 days in low nitrogen (2.4 mM N), high nitrogen (24 mM N) and malt extract (ME) liquid media. After 5 days of incubation in ME medium, transformation of LAS was complete at concentrations < or = 4 mg l(-1), but decreased at higher concentrations. The LAS degradation was not dependent on lignin peroxidases (LiPs) and manganese-dependent peroxidases (MnPs). Mineralization of 14C-ring-LAS to 14CO2 by P. chrysosporium was < 1% regardless of the culture conditions used. Thin layer chromatography and mass spectral analyses indicated that P. chrysosporium transformed LAS to sulfophenyl carboxylates (SPCs) through oxidative shortening of the alkyl side-chains. While LAS disappearance in the cultures was not dependent on LiPs and MnPs, transformation of the parent LAS moieties to SPCs was more extensive in low N medium that favors expression of these enzymes. The SPCs produced in LN cultures were shorter in chain- length than those produced in ME cultures. Also there was a notable shift in the relative abundance of odd and even chain length metabolites compared to the starting LAS particularly in the low N cultures suggesting the possible involvement of processes other than or in addition to beta-oxidation in the chain-shortening process.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12051650</PMID><DateCompleted><Year>2002</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0923-9820</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>6</Issue><PubDate><Year>2001</Year></PubDate></JournalIssue><Title>Biodegradation</Title><ISOAbbreviation>Biodegradation</ISOAbbreviation></Journal><ArticleTitle>Biotransformation of linear alkylbenzene sulfonate (LAS) by Phanerochaete chrysosporium: oxidation of alkyl side-chain.</ArticleTitle><Pagination><MedlinePgn>443-53</MedlinePgn></Pagination><Abstract><AbstractText>The white rot fungus Phanerochaete chrysosporium, which generally mineralizes substituted aromatics to CO2, transformed linear alkylbenzene sulfonate (LAS) surfactants mainly at their alkyl side chain. Degradation of LAS was evidenced by a zone of clearing on LAS-containing agar plates and colorimetric analysis of liquid cultures. Disappearance of LAS was virtually complete within 10 days in low nitrogen (2.4 mM N), high nitrogen (24 mM N) and malt extract (ME) liquid media. After 5 days of incubation in ME medium, transformation of LAS was complete at concentrations < or = 4 mg l(-1), but decreased at higher concentrations. The LAS degradation was not dependent on lignin peroxidases (LiPs) and manganese-dependent peroxidases (MnPs). Mineralization of 14C-ring-LAS to 14CO2 by P. chrysosporium was < 1% regardless of the culture conditions used. Thin layer chromatography and mass spectral analyses indicated that P. chrysosporium transformed LAS to sulfophenyl carboxylates (SPCs) through oxidative shortening of the alkyl side-chains. While LAS disappearance in the cultures was not dependent on LiPs and MnPs, transformation of the parent LAS moieties to SPCs was more extensive in low N medium that favors expression of these enzymes. The SPCs produced in LN cultures were shorter in chain- length than those produced in ME cultures. Also there was a notable shift in the relative abundance of odd and even chain length metabolites compared to the starting LAS particularly in the low N cultures suggesting the possible involvement of processes other than or in addition to beta-oxidation in the chain-shortening process.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yadav</LastName><ForeName>J S</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Microbiology, and NSF Center for Microbial Ecology, Michigan State University, East Lansing 48824-1101, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lawrence</LastName><ForeName>D L</ForeName><Initials>DL</Initials></Author><Author ValidYN="Y"><LastName>Nuck</LastName><ForeName>B A</ForeName><Initials>BA</Initials></Author><Author ValidYN="Y"><LastName>Federle</LastName><ForeName>T W</ForeName><Initials>TW</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>C A</ForeName><Initials>CA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biodegradation</MedlineTA><NlmUniqueID>9100834</NlmUniqueID><ISSNLinking>0923-9820</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013451">Sulfonic Acids</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013451" MajorTopicYN="N">Sulfonic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>6</Month><Day>8</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>6</Month><Day>22</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>6</Month><Day>8</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12051650</ArticleId><ArticleId IdType="doi">10.1023/a:1015056530264</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Federle, T W" sort="Federle, T W" uniqKey="Federle T" first="T W" last="Federle">T W Federle</name><name sortKey="Lawrence, D L" sort="Lawrence, D L" uniqKey="Lawrence D" first="D L" last="Lawrence">D L Lawrence</name><name sortKey="Nuck, B A" sort="Nuck, B A" uniqKey="Nuck B" first="B A" last="Nuck">B A Nuck</name><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Yadav, J S" sort="Yadav, J S" uniqKey="Yadav J" first="J S" last="Yadav">J S Yadav</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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